Reporter

Part:BBa_K1033250:Design

Designed by: Anders Edlund   Group: iGEM13_Uppsala   (2013-09-13)


mCherry fusion protein codon optimised for Lactobacillus reuteri


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene is codon optimised for Lactobacillus reuteri but should work in a wide range of species in the lactobacillus genus. It has been modified according to assembly standard 25 to allow it to be used as a fusion protein.


Source

mCherry has originally been derived from a protein isolated from Discosoma sp. This part has been synthesised and codon optimised for Lactobacillus reuteri by Stefan Roos at SLU in Sweden. It was then biobricked by iGEM Uppsala 2013 according to fusion standard 25.

References